Index to site:
Search our site
Frequently Asked Questions about NTSYSpc
- What is the current version of NTSYSpc? Version 2.2
was initially released Sept. 2005. Note: there will be free service releases (with
numbers and letters like 2.20a) that add new
features and fix problems that were discovered after the initial release
(there will probably be several updates as problems are reported in the new
Please check the Updates page to see if a more recent release is available.
Click on the help|About menu in NTSYSpc to check what release you currently
have installed. While you do not have to uninstall the old version,
it is better to uninstall the old version before the new one is installed.
The uninstall process
will not remove any of your data files.
- Is there a tutorial available? While there is no tutorial guide available, the
printed Getting Started Guide provided with the program gives a step-by-step example. This guide is
also available as an Acrobat PDF file on the distribution CD. It can also be
here (340K). The help file contains step-by-step examples of using NTSYSpc for both a UPGMA cluster analysis and a principal
components analysis. The main problem new users seem to have is due to the expectation
that there is a single button to click to perform an entire analysis. Unlike many
statistical packages, most analyses require one to go through a number of steps. While
less user-friendly, this design makes many more types of analyses possible by combining
the modules in various ways. There is also an install guide that comes with the software.
- End of file or other problems reading a data file? This is the most frequently
reported problem. This almost always is due to an error in preparing the data file.
Common problems are miscounting the number of row or column labels or miscounting
of the number or rows or columns in the matrix itself.
Please double-check your files! Sometimes it helps to have someone else look
at your file.
- Error codes? Did you receive an error message that consists of just a numerical code (such as
"Error 103")? We try to suppress these internal error codes and replace them with meaningful messages.
Some still get through. The help file has a topic that lists all of the error codes.
- Does NTSYSpc read Excel files? NTSYSpc 2.0 did not read
Excel files directly. One had to use the NTedit program to import the file
and save it as a standard NTS file and a copy of the Excel program was required to
be on your computer. Version 2.1 solved that problem by reading Excel files
directly from both NTedit and NTSYSpc. As of release 2.10m all types of
matrices can be read (not just rectangular as in previous releases). A limitation at present is that only
a single matrix can be read from an Excel file (a way around this limitation
is to save your Excel spreadsheet as a CSV format file. Note that Excel and
CSV files must start with NTSYSpc header information. There can be optional comments
and row and column labels. There must be a line that tells NTSYSpc how many rows and columns
to expect. The matrix
header line for Excel files in NTSYSpc ver. 2.0 was slightly different from that of the standard
NTS files. In version 2.1 it is now the same as in the NTS files except that
the suffix codes 'L', 'B', and 'E' must not be used. The NTedit program can now
also write CSV files.
Excel 2007: this version of Excel uses a totally new file format (*.XLSX) that is compatible with NTSYSpc version 2.21c and later. As of version 2.20r, NTSYSpc can read XLS files that Excel 2007 writes in its "compatibility mode." One can also save a spreadsheets as a CSV files for input to NTSYSpc.
- Problems using NTSYSpc? Be sure to check whether a new service
release is available. You will need a password (contact Exeter Software) and
your present registration serial number.
The Updates section of the help file lists the additions, changes, and problems
fixed in each release of NTSYSpc.
- Why does MDSCALE always stops after just 5 iterations even though the solution
is not very good? The default value for "Maximum stress
ratio" was set at "0" (fixed in release 2.10K). You should change that value to something like "0.999".
- Computations of similarity/dissimilarity values seems to 'blow up'?
If a cluster analysis results in no structure and the range of similarity
values going from 0 to 0 or from 1 to 1 then there may be a problem with the
missing value code being set when you did not expect it. Whether or not the
missing value code is set is mentioned in the listing output. In NTedit make
sure the missing value edit box is blank (not a '0') if there are no missing
values in the data.
- Batch codes for MOD3D and MXPLOT. The batch code parameters for specifying
the variables was changed from that in version 1.80 of NTSYSpc.
They now use X, Y and X, Y, Z rather than I, J and I, J, K.
- Need additional copies of the User Guide? There is a PDF file for
the User Guide on the CD you
received from Exeter. You can also view, download, and
print the User Guide from
for version 2.0, this link for version 2.1, or this link for version 2.2..
They are standard Adobe Acrobat PDF files. See question 20 for an installation guide.
- Data size limits? Most modules in NTSYSpc do not have explicit dimension limits for objects or
variables. The limitation will be disk space and time. Larger amounts of RAM
will speed up to computations for very large datasets. With the present capacity
and power of modern PCs, a data set with a few hundred samples or
variables is considered a small dataset for most computations. However, the MDSCALE
module must manipulate many matrices simultaneously and hence is more
limited in the size of the matrix it can handle (512 variables is the
maximum). The canpls module is currently limited to 255 variables.
- Why are only the first 30 OTUs are shown in the dendrogram? By default, a maximum of 30 OTUs
are displayed on each page of the dendrogram. To display additional pages
or to change the number of OTUs/page, right-click on the plot to bring up
the options window. You can change many aspects of the plot including
the fonts used to display the titles and OTU labels. In version 2.1 you can use
the PageUp and PageDown keys to scroll through the other pages. You can also use the "+"
and "-" keys to change the number of OTUs per page.
- Can NTSYSpc use the "," character as a decimal separator? Yes, version 2.20r and later allow a user to specify the decimal character. In the main window, click on Options and then Configure. In the entry form you may change the default "." to a ",". Changing this will also change the separator used in CSV files to a semicolon ";".
- Why doesn't the old DOS version 1.80 of NTSYSpc work on newer computers?
This has puzzled us for some time. Usually an error 200 is encountered.
A solution has been found recently. It turns out the problem is the faster speed of
the newer CPU chips -- not problems with newer Windows versions. A patched
version of the old DOS program is now available (updates).
You will need your original version 1.80 serial/registration number.
- Really stuck on a problem using NTSYSpc? For technical help
to F. James Rohlf, rather than to Exeter Software.
It is usually helpful to attach copies of the files that you are having
problems with so that we can reproduce the problem you are having
and thus get back to you more quickly with a solution.
- A problem was found in the MULREG module. For regression
through the mean, the numerator degrees of freedom for the overall test is k+1
rather than k (where k is the number of independent variables). This was fixed
in version 2.10x. Please update your copy to that version if you plan to use
- Does NTSYSpc work in Windows XP or Vista? Yes - those are the
versions of Windows used for the development of NTSYSpc.
- How should I cite NTSYSpc in a publication? You can use the following
format: Rohlf, F. J. 2008. NTSYSpc: Numerical Taxonomy System, ver. 2.20.
Exeter Publishing, Ltd.: Setauket, NY. [substitute, of course, the version
number you have and its copyright date from the Help|About window.]
- What happened to the ntsys.ini file? Release 2.20w has made an important change in the placement of the ntsys.ini file. Earlier versions placed it in the same directory as the ntsys.exe file. This caused problem on some computers (such as student labs where strict constraints are placed on where a user can store files). Release 2.20w now places the file in an ntsys subdirector under the current user's My Documents directory. This change should make the network version of NTSYSpc unnecessary (but it still will be made available for a while).
The following text applies to earlier releases: Release 2.11L N and later makes an important
change in the location of the ntsys.ini file in the network version of NTSYSpc. It is placed in the the
NTSYSpc directory within a user's Document and Settings directory rather than
in the same directory as the program file. This change allows different users
of the same computer to have their own copies of the ntsys.ini file. It also
makes it possible to use NTSYSpc with the ntsys.exe file located on a server.
A limitation of this change is that you may receive an error message such as
the following if you do not have Windows 98 or higher, version 4.0 or later of MS Internet Explorer,
or you have not installed its "Desktop update". Version 4.71 or
later of SHELL32.DLL is now required. Please contact us if this is a problem.
More information about the MS "desktop update" can be found at http://support.microsoft.com/default.aspx?scid=kb;en-us;290557.
- Why do the numbers in the output file seem to be off by a factor of 10?
NTSYSpc uses an exponential format in the output files so as to preserve as
much accuracy as possible. The numbers are in the format x.xxxxxxxxxeyyy where
the "yyy" indicates the power of 10 to multiply the "x.xxxxxxxxx".
Thus, for example, "2.00000000000000E-001" corresponds to
"0.2", "1.05477465476420E+002" corresponds to "105.477465476420",
and "1.02237799184597E+000" to "1.02237799184597" .
- Is there a step by step guide to the installation of NTSYSpc?
Yes, a guide is
available as a PDF file here.
- When using older versions of NTSYSpc on Vista or Windows 7, there
will be an error
message "There was a problem running the macro. (1037)" when trying to view the
help file. The help file is still functional but the index and search
features are not available. Release 2.20n and more recent use the HTMLhelp file format
(ntsys.chm file) rather than the older WinHelp (ntsys.hlp) format to solve this
This file was last modified on 15 July 2010.